Fig. 4
From: Enrichment and proteomic identification of Cryptosporidium parvum oocyst wall
Expression of CpSP1 in Escherichia coli. A PCR amplification of the Cpcgd7_5140 gene of C. parvum. Lane M: 2000-bp DNA ladder marker. Lane 1: Cpcgd7_5140 product. B Analysis of CpSP1 expressed in E. coli and purity of recombinant CpSP1 revealed by SDS-PAGE. Lane 1: lysate from bacterial cells transformed with pGEX-4t-1-Cpcgd7_5140 without IPTG induction. Lane 2: lysate from bacterial cells transformed with pGEX-4t-1-Cpcgd7_5140 with IPTG induction. Lane 3: CpSP1 purified using gradient urea and PD-10 desalting columns. C Western blot analysis of recombinant CpSP1 in E. coli using Anti-GST tag. Lane M: protein size markers. Lane 1: lysate from bacterial cells transformed with pGEX-4t-1-Cpcgd7_5140 with IPTG induction