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Fig. 6 | Parasites & Vectors

Fig. 6

From: A novel 2B4 receptor leads to worse pregnancy outcomes by facilitating TNF-α and IFN-γ production in dNK cells during Toxoplasma gondii infection

Fig. 6

In human dNK cells, the 2B4/SHP-2/p-P38 pathway is involved in 2B4-induced downregulation of TNF-α and IFN-γ after T. gondii infection. A Some inhibitors may induce cell death, so 7-AAD was used to exclude the effect of SHP-2 inhibitor and Fyn inhibitor on dNK cells. B SHP-2 expression in uninfected and infected dNK cells was assessed by PCR (data are presented as the mean ± SD, n = 3, *P < 0.05, by the paired t-test). C SHP-2, p-SHP-2, and Fyn expression in uninfected and infected dNK cells as assessed by Western blot (data are presented as the mean ± SD, n = 3, *P < 0.05, **P < 0.01, by the paired t-test). D Expression of SHP-2, Fyn, p-ERK, p-P38, and 2B4 in infected dNK cells with or without anti-2B4 antibody as assessed by Western blot. E Expression of p-P38, p-ERK, TNF-α, IFN-γ, and 2B4 in the infected group with or without anti-2B4 antibody (data are presented as the mean ± SD, n = 3, *P < 0.05, by the paired t-test). F Levels of p-2B4, p-P38, p-ERK, TNF-α, and IFN-γ in the infected group with or without SHP-2 inhibitor treatment. G Expression of SHP-2, p-P38, p-ERK, TNF-α, and IFN-γ in uninfected, infected, infected and treated with a 2B4 antibody groups, and infected with 2B4 antibody and SHP-2 inhibitor groups. H Analysis for Fig. 6g (data are presented as the mean ± SD, n = 3, *P < 0.05, by the paired t-test). I Input for SHP-2, 2B4, p-P38, IFN-γ, and TNF-α when we used SHP-2 antibody to pull down 2B4 and p-P38. j 2B4 and p-P38 were synchronously immunoprecipitated by SHP-2 antibody

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