Fig. 7

In human dNK cells, the 2B4/Fyn/p-ERK pathway is involved in 2B4-induced downregulation of TNF-α and IFN-γ after T. gondii infection. A Fyn, p-ERK, TNF-α, p-P38, and IFN-γ expression in the infected group with or without Fyn inhibitor treatment. B Expression of p-ERK, TNF-α, p-P38, and IFN-γ in the infected group, infected group treated with the 2B4 antibody, and the infected group treated with both anti-2B4 antibody and the Fyn inhibitor. C Analysis for Fig. 7b (data are presented as the mean ± SD, n = 3, **P < 0.01, *P < 0.05, by the paired t-test). D Input for Fyn, p-ERK, 2B4, IFN-γ, and TNF-α when we used Fyn to bind 2B4 and p-ERK. E 2B4 and p-ERK were synchronously immunoprecipitated by Fyn antibody. F Input for 2B4, SHP-2, Fyn, TNF-α, and IFN-γ when we used 2B4 to bind to SHP-2 or Fyn. G SHP-2 and Fyn were synchronously immunoprecipitated by 2B4 antibody. H Expression of TNF-α and IFN-γ in infected dNK cells with or without p-ERK inhibitor or p-P38 inhibitor, respectively. I Analysis of Fig. 7h (data are presented as the mean ± SD, n = 3, *P < 0.05, by the paired t-test)