Fig. 1
Live N. caninum tachyzoites inhibit established B16F10 melanoma in mice. a Mice experiment schedule: B16F10 cells (2 × 105) were inoculated subcutaneously on the left flank of C57BL/6 mice, and intratumoral or distal subcutaneous injections of N. caninum were given when tumor diameter reached 3–5 mm, at a dose of 2 × 106 tachyzoites per mouse. b The macroscopic size of tumors in each group of mice on day 21 (n = 5). c Quantification of the dissected tumor volume on day 21 for each treatment group (mean ± SD; n = 5). d Comparison of tumor volume in tumor-bearing mice (n = 5) treated with PBS or N. caninum tachyzoites. Data were analyzed by the two-tailed Student t-test (c). *P < 0.05, **P < 0.01, ***P < 0.001. Abbreviations: B16F10 + PBS, tumor control group; intratumorally, intratumoral injection; subcutaneously, distal subcutaneous injection; subcutaneous injection, distal subcutaneous injection