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Fig. 2 | Parasites & Vectors

Fig. 2

From: Periviscerokinin (Cap2b; CAPA) receptor silencing in females of Rhipicephalus microplus reduces survival, weight and reproductive output

Fig. 2

Rhimi-CAP2bR RNAi silencing using the double-stranded RNAs (dsRNAs) ds680-805, ds956-1109 and ds1102-1200. a Rhimi-CAP2bR silencing evaluation by qRT-PCR for whole body of partially fed females, 3 days on animals. Different lowercase letters above bars indicate significant difference at P < 0.05), based on statistical analysis using one-way ANOVA followed by a Tukey’s multiple comparisons test. b Percentage of mortality of females treated with dsRNA ds680-805, ds956-1109 or ds1102-1200 up to the assay endpoint. Different lowercase letters above graph indicate significant difference at P < 0.05), based on statistical analysis using one-way ANOVA followed by a Tukey’s multiple comparisons test. Between three and nine (n = 3–9) biological replicates were analyzed per treatment. dsβ-act, dsRNA R. microplus beta-actin-injected (positive control) ticks; dsβ-lac, dsRNA beta-lactamase-injected (negative control) ticks; NI, non-injected ticks

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