Periviscerokinin (Cap2b; CAPA) receptor silencing in females of Rhipicephalus microplus reduces survival, weight and reproductive output

Wulff, Juan P.; Temeyer, Kevin B.; Tidwell, Jason P.; Schlechte, Kristie G.; Lohmeyer, Kimberly H.; Pietrantonio, Patricia V.

doi:10.1186/s13071-022-05457-7

Table 1 Oligonucleotide primers for cloning, double-stranded RNA synthesis and quantitative reverse-transcriptase PCR

From: Periviscerokinin (Cap_2b; CAPA) receptor silencing in females of Rhipicephalus microplus reduces survival, weight and reproductive output

Primer name	Oligo sequence 5′- 3′^a	Notes
RmPerivis-200U19	ATCTGCTGTGCCGACACTG	Rhimi-CAP_2bR PCR
RmPerivis-1716L21	GGACAGGGTATGGCGTTTATG	Rhimi-CAP_2bR PCR
RmPerivis-388L18	CCGTATGGTGGCGTTGTC	5’-RACE-PCR
RmPerivis-195L20	GTCGGCACAGCAGATAGTCC	5’-RACE-PCR
Long-Univ.-RACE	CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT	RACE-PCR
Short-Univ.-RACE	CTAATACGACTCACTATAGGGC	RACE-PCR
RmPeriviscR-680U20	CCCGCTTCAAGCTGCATGTG	Rhimi-CAP_2bR dsRNA synthesis
RmPeriviscR-805L20	CCCGTCCACCATGCACTTTA	Rhimi-CAP_2bR dsRNA synthesis
RmPeriviscR-680U20-T7	AAAGGCCTTAATACGACTCACTATAGGCCCGCTTCAAGCTGCATGTG	Rhimi-CAP_2bR dsRNA synthesis
RmPeriviscR-805L20-T7	AAAGGCCTTAATACGACTCACTATAGGCCCGTCCACCATGCACTTTA	Rhimi-CAP_2bR dsRNA synthesis
RmPeriviscR-956U20	GTGAAGGTTTCGCTTGAAGA	Rhimi-CAP_2bR dsRNA synthesis
RmPeriviscR-1109L20	CCCTTCACAGTGTCGGCACA	Rhimi-CAP_2bR dsRNA synthesis
RmPeriviscR-956U20-T7	AAAGGCCTTAATACGACTCACTATAGGTGAAGGTTTCGCTTGAAGA	Rhimi-CAP_2bR dsRNA synthesis
RmPeriviscR-1109L20-T7	AAAGGCCTTAATACGACTCACTATAGGCCCTTCACAGTGTCGGCACA	Rhimi-CAP_2bR dsRNA synthesis
RmPeriviscR-1102U20	TATCTGCTGTGCCGACACTG	Rhimi-CAP_2bR dsRNA synthesis
RmPeriviscR-1200L20	CAGCACCGCAAGGATAATCA	Rhimi-CAP_2bR dsRNA synthesis
RmPeriviscR-1102U20-T7	AAAGGCCTTAATACGACTCACTATAGGTATCTGCTGTGCCGACACTG	Rhimi-CAP_2bR dsRNA synthesis
RmPeriviscR-1200L20-T7	AAAGGCCTTAATACGACTCACTATAGGCAGCACCGCAAGGATAATCA	Rhimi-CAP_2bR dsRNA synthesis
Amp-fwd	CGCTGGTGAAAGTAAAATATG	beta-lactamase dsRNA synthesis [29]
Amp-rev	GCCGGGAAGCTAGAGTAAGTA	beta-lactamase dsRNA synthesis [29]
Amp-T7	AAAGGCCTTAATACGACTCACTATAGGCGCTGGTGAAAGTAAAATATG	beta-lactamase dsRNA synthesis [29]
Amp-T7	AAAGGCCTTAATACGACTCACTATAGGCCGGGAAGCTAGAGTAAGTA	beta-lactamase dsRNA synthesis [29]
BmBActin-1U20	TCCTCGTCCCTGGAGAAGTC	Rhimi-ACTB dsRNA synthesis [29]
BmBActin-285L18	GGGGGAGCGATGATCTTG	Rhimi-ACTB dsRNA synthesis [29]
BmbActin-1U20-T7	AAAGGCCTTAATACGACTCACTATAGGTCCTCGTCCCTGGAAGAAGTC	Rhimi-ACTB dsRNA synthesis [29]
BmbActin-285L18-T7	AAAGGCCTTAATACGACTCACTATAGGGGAGCGATGATCTTG	Rhimi-ACTB dsRNA synthesis [29]
RmCAP2b-qF1	ATGCGGGCCCTCATCAT	Rhimi-CAP_2bR qRT-PCR [25]
RmCAP2b-qR1	AATGGCGGTTCCTGGTTAGG	Rhimi-CAP_2bR qRT-PCR [25]
BmELF1a-88-F	CGTCTACAAGATTGGTGGCATT	Rhimi-EF1A qRT-PCR [57]
BmELF1a-196-R	CTCAGTGGTCAGGTTGGCAG	Rhimi-EF1A qRT-PCR [57]
RmRPS4-qF1	TCATCCTGCACCGCATCA	Rhimi-RPS4 qRT-PCR [27]
RmRPS4-qR1	ACGCGGCACAGCTTGTACT	Rhimi-RPS4 qRT-PCR [27]

cDNA Complementary DNA, dsDNA double-stranded DNA, qRT-PCR quantitative reverse-transcriptase PCR, RACE rapid amplification of cDNA ends, Rhimi-CAP_2bR Rhipicephalus microplus periviscerokinin receptor
^aSequences in italics were added to the primers and are not part of the tick cDNA sequences

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ISSN: 1756-3305

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