Fig. 2

Summary of the experimental design and sample size of wild-caught Anopheles scanned to determine NIRS accuracy for predicting Anopheles species according to preservation methods. Using silica gel as preservative, the model was trained taking into account 85 Anopheles mosquitoes (42.5 An. gambiae and 42.5 An. coluzzii). The trained model was validated using 42 Anopheles and tested on 42 Anopheles (21 An. gambiae and 21 An. coluzzii). With ethanol as preservative, the model was trained using 78 Anopheles mosquitoes (39 An gambiae and 39 An. coluzzii) per replication. This model was validated using 39 Anopheles and tested on 39 Anopheles mosquitoes (19.5 An. gambiae and 19.5 An. coluzzii)