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Fig. 3 | Parasites & Vectors

Fig. 3

From: A novel cysteine protease inhibitor in Baylisascaris schroederi migratory larvae regulates inflammasome activation through the TLR4–ROS–NLRP3 pathway

Fig. 3

rBsCPI-1 affects NLRP3 activation through the TLR4-ROS-NLRP3 pathway. A The fluorescence intensity of intracellular fluorogenic dye 2′-7′dichlorofluorescin diacetate (DCFH-DA) was detected at 488 nm excitation and 525 nm emission wavelengths. The data presented are representative of 3 independent experiments. Significant differences versus the control group (PBS + PBS) are indicated by *P < 0.05, ***P < 0.001; for the LPS + CPI-1 group versus the LPS + PBS group by &&&P < 0.001; for the 7E3 + CPI-1 group versus the 7E3 + PBS group by #P < 0.05; and for the LPS + CPI-1 group versus the LPS + PBS group by &&P < 0.01. B, C A representative western blot is shown in B, and the graph of the quantified band density is shown in C. C *P < 0.05; versus the NAC + PBS group by §P < 0.05; versus PBS + CPI-1 group by &&&P < 0.001. D Fluorescence intensity of intracellular DCFH-DA. Significant differences versus the control group are indicated by *P < 0.05, **P < 0.01, ***P < 0.001; for the MCC 950 + CPI-1 group or Nigericin + CPI-1 group versus the PBS + CPI-1 group by §P < 0.05; for the Nigericin + CPI-1 group versus the Nigericin + PBS group by &P < 0.05. 7E3, TLR-4-blocking antibody; LPS, lipopolysaccharide (TLR4 activator); MCC 950, MCC950 Sodium (a NLRP3 inhibitor); NAC, N-acetylcysteine (a ROS inhibitor); ROS, reactive oxygen species; TLR4, Toll-like receptor 4

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