Fig. 3
Sensitivity of the RPA-CRISPR-Cas12a detection platform. a Sensitivity of the RPA-CRISPR-Cas12a platform to fluorometric assays using recombinant plasmids. TV-actin−/− was used as a negative control. b RPA-CRISPR detected 100 copies of the actin gene by the Cas12a excision reaction within 60 min (n = 3; ****P < 0.0001; **P < 0.01; bars represent the means ± standard error of the mean). c Sensitivity of the RPA-CRISPR-Cas12a platform to the LFS sensor using recombinant plasmids. NC was used as a negative control. d Sensitivity test of the fluorescence assay using crude DNA extracted from T. vaginalis. e RPA-CRISPR detected one T. vaginalis by Cas12a excision reaction within 60 min (n = 3; ****P < 0.0001; ***P < 0.01 bars represent the means ± standard error of the mean). f Sensitivity test of crude DNA extracted from T. vaginalis by the LFS sensor