Fig. 5

Genotyping and virus determination of the T. vaginalis standard strain and clinical strains. a Agarose gel electrophoresis for genotyping of the T. vaginalis standard strain. b Agarose gel electrophoresis for genotyping of the sample 18. c Agarose gel electrophoresis for genotyping of sample 4; actin was a nonenzymatic control. d Agarose gel electrophoresis for genotyping sample 22. e Phylogenetic tree of the actin gene of T. vaginalis. The sequences detected in this study are indicated by the diamond shape. The other sequences shown in the tree were reference genotypes collected from GenBank. The scale bar shows the nucleotides per 1000 substitutions. f Agarose gel electrophoresis of total RNA of the T. vaginalis standard strain (TVV^−/−). g Agarose gel electrophoresis of total RNA of clinical isolate 4 (TVV)