Table 1 Serological tests and antigens used in studies with dogs and cats

EIA

Whole lysate extracted from epimastigotes of Colombian T. cruzi strains Cas-15 and Gal-61

No

Serum

D (n = 251)

[178]

IFAT

Complete epimastigotes of Colombian T. cruzi strains Cas-15 and Gal-61

IHA

Chagastest HAI test (Wiener Laboratories Rosario, Argentina)

EIA

TSSApep lineage-specifc

No

Serum

D (n = 85)

C (n = 19)

A (n = 7)

[179]

RDT

Chagas Sero K-SeT RDT

EIA

Laboratorio-Lemos SRL, Buenos Aires, Argentina

No

Serum

D (n = 291)

[32]

IHA

Polychaco, Laboratorio-Lemos SRL, Buenos Aires, Argentina

IFAT

According to [180]—(Tulahuén strain)

No

Serum

D (n = 54)

[47]

EIA

ELISA of Biozima kit (Polychaco)

IHA

Hemacruzi (BioMerieux)

IFAT

According to [180]—(Tulahuén strain)

No

Serum

D (n = 182)

[181]

IHA

Polychaco S.A.I.C, Buenos Aires, Argentina

EIA

Homogenate of the flagellar fraction of T. cruzi

IFAT

Complete epimastigotes of F90 and Y88 strains

No

Serum

D (n = 330)

[65]

EIA

Chimeric recombinant proteins IBMP

RDT

InBios Stat-Pak rapid test

No

Serum

C (n = 284)

[124]

EIA

T. cruzi parasite lysate from strain WB1

RDT

Trypanosoma Detect2 MRA Rapid Test. Inbios International Ltd., Washington, USA

No

Serum

D (n = 67)

[85]

EIA

Whole lysate extracted from epimastigotes of NC-9 strain

No

Serum

D (n = 2)

[182]

IFAT

Complete epimastigotes of NC-9 strain

IFAT

Complete epimastigotes of Panamanian Burunga strain

No

Serum

D (n = 99)

[92]

EIA

ELISA Chagastest. Wiener Lab., Argentina modified with whole lysate extracted from epimastigotes of Panamanian Burunga strain

EIA

Iron superoxide dismutase—FeSODe

No

Serum

D (n = 303)

[41]

WB

Iron superoxide dismutase—FeSODe

IFAT

According to [180]

Yes

Serum

D (n = 481)

[183]

IHA

Polychaco SAIC, Buenos Aires, Argentina

CFT

According to [184]

DAT

Polychaco SAIC, Buenos Aires, Argentina

IHA

Polychaco, Buenos Aires, Argentina

No

Serum

D (n = 86)

C (n = 38)

[17]

EIA

Homogenate of the flagellar fraction of T. cruzi

IFAT

According to [180]—(Tulahuén strain)

RDT

Trypanosoma Detect, Inbios, Washington, USA

Yes

Serum

D (n = 199)

C (n = 57)

[185]

IHA

Polychaco, Buenos Aires, Argentina

EIA

ELISA A: anti-IgG-HRP; Santa Cruz Biotechnology, Santa Cruz, CA

ELISA B: using recombinant trans-Sialidase as antigen

IFAT

Fluorescein-conjugated anti-gammaglobulin LID; Laboratorio Inmunodiagnóstico, Buenos Aires, Argentina

WB

TESA-blotting, BioMerieux based on strain Y

No

Serum

D (n = 111)

[83]

RDT

Chagas StatPak^® Assay, Chembio, USA

No

Serum

D (n = 153)

[86]

EIA

Total proteins from Querétaro strain of T. cruzi

No

Serum

D (n = 209)

[42]

WB

Total proteins from Querétaro strain of T. cruzi

IFAT

Complete epimastigotes of Colombian T. cruzi strains I00/BR/00F (TcI) and MHOM/BR/1957/Y (TcII)

No

Serum

D (n = 62)

WM (n = 36)

[64]

EIA

ELISA, Bio-Manguinhos, Rio de Janeiro, Brazil

EIA

Plates of Chagas III ELISA kit (Grupo Bios^®) and monoclonal secondary antibody goat anti-dog IgG-HRP: sc-2433 (Santa Cruz Biotechnology, INC)

No

Serum

D (n = 356)

[48]

IFAT

N.I.H.—Colombia

RDT

Chagas Sero K-SeT (TSSA peptide epitope specific to TcII/V/VI)

Yes

D (n = 57)

[172]

RIPA

Tulahuén strain epimastigote lysate

No

Serum

D (n = 301)

[57]

RDT

Chagas Stat-Pak (Chembio, Medford, NY, USA)

No

Serum

D (n = 540)

[56]

EIA

Whole parasite lysate from a local strain WB1

WB

Whole parasite lysate from a local strain WB1

RDT

Chagas Stat-Pak (Chembio, Medford, NY, USA)

Chagas Detect Plus Rapid Test (InBios International, Inc., WA, USA)

No

Serum

C (n = 167)

[12]

IFAT

Texas Veterinary Medical Diagnostic Laboratory (TVMDL, College Station, TX)

EIA

Recombinant proteins PGR31-His, PGR30-His and PGR24-His

No

Serum

D (n = 333)

[93]

MABA

Recombinant proteins PGR31-His, PGR30-His and PGR24-His

EIA

Modified Gold ELISA Chagas commercial test Chimeric recombinant proteins IBMP

No

Serum

Dogs (n = 40)

[69]