Table 2 Specific oligonucleotide names and primer sequence targets selected for pathogen qPCR amplification
Target organism | Oligonucleotide name | Oligonucleotide sequence (5′-3′) | Target gene | PCR product size (bp) | References |
|---|---|---|---|---|---|
Bartonella spp. | Bart_ssrA_F | GCTATGGTAATAAATGGACAATGAAATAA | ssrA | 158 | [92] |
Bart_ssrA_R3 | GACAACTATGCGGAAGCACGTC | ||||
Bartonella spp. | BsppITS325s | CCTCAGATGATGATCCCAAGCCTTCTGGCG | ITS | 130 | [87] |
BsppITS543as | AATTGGTGGGCCTGGGAGGACTTG | ||||
BsppITS500p | FAM-GTTAGAGCGCGCGCTTGATAAG- IABkFQ | ||||
Rickettsia spp. | Rick23-5_F2 | AGCTCGATTGATTTACTTTGCTG | 23S-5S | 247 | [92] |
Rick23-5_R | TTTGTATTGCTAGCTTGGTGG | ||||
R. felis | RifelisOmpA-172Â s | AGTCCTTGGTGCTGCAAGAACCGTAACTG | OmpA | 160 | This study |
RifelisOmpA-330as | ACCACTGAACCTAATGAAATATCACCAGT | ||||
R. asembonensis | RiasemboOmpA-175Â s | GTTGGGAGGAACAACGATAGATGCA | OmpA | 120 | This study |
RiasemboOmpA-245as | ACCGTAAATAAACCAGGAGCAAAACCA | ||||
Mycoplasma spp. | Myco_Hf_F.1 | GACGAAAGTCTGATGGAGCAAT | 16S rRNA | 127 | [13] |
Myco_Hf_R | ACGCCCAATAAATCCGRATAAT | ||||
Anaplasma and Ehrlichia spp. | AE16S_45F | AGCYTAACACATGCAAGTCGAACG | 16S rRNA | 199 | [90] |