Fig. 5

Transcript level determination of candidate GlMyb2 regulons identified by ChIP-seq. Both WB and JK2 strains grown in an encystation medium for 3, 6, and 14 h were harvested for RNA extraction. The transcript levels of 22 candidate genes were monitored in both WB (open circles) and JK2 (closed circles) by quantitative PCR using gene-specific primers. For each gene transcript levels were normalized to those of actin (GL50803_15113). Each gene is indicated by the gene number in a state of omission of GL50803_, provided by the Giardia database, and its putative function. a GlMyb2 target genes showing GlMyb2-dependent and encystation-induced expression. *P = 0.01–0.05, **P < 0.01. b Candidate genes of which expression is independent of encystation and/or GlMyb2. Three independent replicates, each consisting of three technical replicates, were evaluated. Error bars represent the standard error of the mean. c Schematic diagrams showing consensus GlMyb2-binding sites in the promoters of the identified GlMyb2 target genes