Groups | Number of rabbits | Immunogen and dosage | Vaccination weeks | Vaccination route | infection dose/week/route |
---|---|---|---|---|---|
PBS-uninfected | 10 | 1 ml Sterile PBS | 0, 2 | Subcutaneous injection in the neck | – |
PBS-infected | 10 | 1 ml Sterile PBS | 0, 2 | Subcutaneous injection in the neck | 1 × 104 sporulated oocysts/week 4/oral |
Quil-A-infected | 10 | 1 mg Quil-A dilution in 1 ml PBS | 0, 2 | Subcutaneous injection in the neck | 1 × 104 sporulated oocysts/week 4/oral |
Trx-His-S-infected | 10 | 100 μg Trx-His-S tag + 1 mg Quil-A dilution in 1 ml PBS | 0, 2 | Subcutaneous injection in the neck | 1 × 104 sporulated oocysts/week 4/oral |
rEsIMP1 | 10 | 100 μg rEsIMP1 + 1 mg Quil-A dilution in 1 ml PBS | 0, 2 | Subcutaneous injection in the neck | 1 × 104 sporulated oocysts/week 4/oral |
rEsAMA1 | 10 | 100 μg rEsAMA1 + 1 mg Quil-A dilution in 1 ml PBS | 0, 2 | Subcutaneous injection in the neck | 1 × 104 sporulated oocysts/week 4/oral |