Fig. 9

AgB intervention ameliorated the damage due to macrophages in vitro. A RAW264.7 cells were used to test the effect of AgB on macrophage differentiation (×200). B Macrophages were incubated with 1000 ng/ml AgB, and after 1 h, the cells were stimulated by the addition of LPS/IFN-γ. After 20 h, the amount of NO present in the cultured cell supernatant was measured using the nitric acid reduction method. In the LPS+AgB group, the level of NO was significantly decreased (178.11 ± 22.63) compared with the LPS group (243.18 ± 16.45). Comparison between the groups was performed using one-way ANOVA for statistical analysis (the experiment was repeated independently, n = 5, ∗ ∗ P < 0.01)