Fig. 2
Expression of pyroptosis-related molecules in vivo. a Gene expression of pyroptosis-related molecules, NLRP3, ASC, pro-caspase-1, and GSDMD at mRNA levels in the lymph node of infected mice. Mice were infected with RHwx2−/−, RH, RHwx2+/+ strain, respectively, then total RNA was extracted, and RT-qPCR was performed with the indicated primer. b Protein expression of pyroptosis-related molecules of NLRP3, caspase-1, caspase-1p20, GSDMD-FL, GSDMD-N, IL-1β, IL-1β p17 in lymph node of mice infected with the RHwx2−/−, RH, and RHwx2+/+ strains, and western blot was performed with the indicated antibody. c Analysis of grayscale scanning of image B