Table 1 Information of primers
Name of primer | Forward (5′-3′) | Reverse (5′-3′) |
|---|---|---|
wx2-CDS | ATGTATATCTGTATAGAAGG | CGGTGTCGCCTGACTTCTGT |
pTub-Gbison | TACCCGTACGACGTCCCGGACTACGCTGGCTATCCC | TTTGTCGAAAAAGGGAATTCAAGAAAA |
Com-UPRT-wx2-KZ | TCCTTTTATTCCAAGATCTGTGGCGTCTCGATTGTGAGGAAGTGGAGGACGGGAATTCG | AAACTGCCCGCAAGCCACTTTCCATCGACTCGCCAGCTAATACGACTCACTATAGGGCG |
JD1-wx2 | TCCGTAAAGCGGTGAGTGTCG | CGGTGTCGCCTGACTTCTGTG |
JD2-wx2 | AAACATCCGTAAAGCGGTGAG | ACGACGAAGAAGGGAACACG |
pro-caspase-1 | CACAGCTCTGGAGATGGTGA | CTTTCAAGCTTGGGCACTTC |
ASC | GACAGTACCAGGCAGTTCGT | AGTCCTTGCAGGTCAGGTTC |
pro-IL-1β | CAGGCAGGCAGTATCACTCA | AGCTCATATGGGTCCGACAG |
NF-κB | GAGGAAGGCTGTGAACATGAGG | TTCTGGTGCATTCTGACCTTGC |
NLRP3 | AGATTACCCGCCCGAGAAAG | TCCCAGCAAACCCATCCACT |
β-actin | TTCCTTCCTTGGGTATGGAAT | GAGCAATGATCTTGATCTTC |