Fig. 1

a–h Expression of T cell immunoglobulin and mucin domain 3 (Tim-3) on human and murine decidual dendritic cells (dDCs) after Toxoplasma gondii infection. a The expression of Tim-3 on myeloid DCs (CD11c⁺CD8α⁻) from uninfected and infected mice was examined by flow cytometry. b The changes in Tim-3 levels in the uninfected and infected groups were statistically analyzed. c The expression of Tim-3 on the surface of lymphatic DCs (CD11c⁺CD8α⁺) from uninfected and infected mice was examined by flow cytometry. d The changes in Tim-3 levels between the uninfected and infected groups were statistically analyzed. e The expression of Tim-3 on myeloid DCs (lineage⁻ HLA–DR⁺) from uninfected and infected human decidua was examined by flow cytometry. f The changes in Tim-3 expression in the uninfected and infected groups were statistically analyzed. g The expression of Tim-3 on human myeloid DCs from the uninfected and infected groups was examined by western blotting. h The changes in Tim-3 expression between the uninfected and infected groups were statistically analyzed. The data shown are the means ± SD. At least six pregnant mice in each group were assayed individually, and differences in the results were identified by unpaired t-test: uninfected vs. infected group. At least six samples of human decidua in each group were assayed individually by flow cytometry, and at least three samples of human decidua in each group were assayed individually by western blotting. Differences in the results were identified by paired t-test. * P < 0.05, ** P < 0.01, *** P < 0.001