Fig. 2

a Impact of Tim-3 on adverse pregnancy outcomes caused by Toxoplasma gondii infection in mice. a The mental states of uninfected pregnant mice were good, and the fetal mice developed well. b Pregnant mice infected with T. gondii showed listlessness, and the placentas and fetuses exhibited inflammatory congestion. c Tim-3-deficient (Tim-3^−/−)-infected pregnant mice had worse mental states, more stillbirths, and their fetuses were absorptive or stunted. d The weights of the placentas and fetuses and the abnormal rates in uninfected, infected and infected Tim-3^−/− pregnant mice were statistically analyzed. e–g The developmental state of fetuses was observed by scanning electron microscopy. h–j Placentas from uninfected, infected, and infected and pregnant Tim-3^−/− mice were stained with hematoxylin and eosin (H&E) and showed severe bleeding, erythrocyte aggregation and cellular necrosis (black circle). At least three pregnant mice in each group were assayed individually, and differences in the results identified by unpaired t-test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001