Fig. 4

a–f Tim-3 downregulation on human and murine dDCs by Toxoplasma gondii infection is associated with the expression of indoleamine 2,3-dioxygenase (IDO) and interleukin-10 (IL-10). The expression of IDO (a) and IL-10 (b) on myeloid and lymphoid dDCs from uninfected, infected, and infected and Tim-3^−/− mice was examined by flow cytometry and the results statistically analyzed. The expression of IDO (c) and IL-10 (d) on human myeloid dDCs from the uninfected, infected and Tim-3-neutralized plus infected groups was examined by flow cytometry and statistically analyzed. The levels of IDO (e) and IL-10 (f) in human dDCs from the uninfected, infected and Tim-3-neutralized infected groups were examined by western blotting. The data shown are the means ± SD. At least six samples of human decidua in each group were assayed individually by flow cytometry, and at least three samples of human decidua in each group were assayed individually by western blot. Differences in the results were identified by paired t-test. At least six pregnant mice in each group were assayed individually, and differences in the results identified by unpaired t-test: uninfected vs. infected group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001