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Fig. 5 | Parasites & Vectors

Fig. 5

From: Tim-3 downregulation by Toxoplasma gondii infection contributes to decidual dendritic cell dysfunction

Fig. 5

a–i Tim-3 downregulation on human and murine dDCs after Toxoplasma gondii infection is associated with the expression of interleukin-12 (IL-12) and tumor necrosis factor-α (TNF-α). The levels of IL-12 (a) and TNF-α (c) on myeloid and lymphoid dDCs from uninfected, infected, and infected and Tim-3−/− mice were analyzed by flow cytometry. The levels of IL-12 (b) and TNF-α (d) on myeloid and lymphoid dDCs from uninfected, infected, and infected and Tim-3−/− mice were statistically analyzed. e The expression of TNF-α on human myeloid dDCs from uninfected, infected, and Tim-3-neutralized plus infected groups was analyzed by flow cytometry. h The levels of IL-12 on human myeloid dDCs from uninfected, infected and Tim-3-neutralized plus infected groups was analyzed by western blotting. f, i The expression of TNF-α and IL-12 in human dDCs was statistically analyzed. The data shown are the means ± SD. At least six samples of human decidua in each group were assayed individually by flow cytometry, and at least three samples of human decidua in each group were assayed individually by western blotting. Differences in the results were identified by paired t-test. At least six pregnant mice in each group were assayed individually, and differences in the results identified by unpaired t-test: uninfected vs. infected group. * P < 0.05, ** P < 0.01, *** P < 0.001

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