Table 1 Comparative detection of Dirofilaria immitis in dogs from 11 communities in south Texas, USA, using different diagnostic assays
Coloniaa | No. of samples tested | No. of samples antigen positive pre-ICD (%) | No. of samples tested | No. of samples antigen positive post-ICD (%) | No. of samples tested | No. of samples HRM qPCR positive (%) | No. of samples tested | No. of samples probe-based qPCR positive |
|---|---|---|---|---|---|---|---|---|
A | 17 | 6 (35.3) | 17 | 8 (47.1) | 17 | 0 (0.0) | 17 | 4 (23.5) |
M | 5 | 1 (20.0) | 5 | 4 (80.0) | 5 | 0 (0.0) | 5 | 1 (20.0) |
N | 7 | 3 (42.9) | 7 | 3 (42.9) | 7 | 0 (0.0) | 7 | 2 (28.6) |
O | 25 | 10 (40.0) | 25 | 11 (44.0) | 25 | 0 (0.0) | 24b | 7 (29.2) |
P | 17 | 10 (58.8) | 17 | 10 (58.8) | 12b | 0 (0.0) | 12b | 3 (25.0) |
Q | 42 | 9 (21.4) | 42 | 10 (23.8) | 34b | 1 (2.9) | 30b | 5 (16.7) |
R | 19 | 9 (47.4) | 19 | 10 (52.6) | 19 | 0 (0.0) | 13b | 4 (30.8) |
S | 23 | 12 (52.2) | 23 | 15 (65.2) | 0b | NA | 0b | NA |
U | 15 | 0 (0.0) | 14a | 2 (14.3) | 15 | 0 (0.0) | 15 | 0 (0.0) |
V | 16 | 6 (37.5) | 16 | 7 (43.8) | 16 | 0 (0.0) | 16 | 3 (18.8) |
T | 14 | 1 (7.1) | 14 | 1 (7.1) | 8b | 0 (0.0) | 10b | 1 (10.0) |
Total | 200 | 67 (33.5) | 199 | 81 (40.7) | 158 | 1 (0.06) | 149 | 30 (20.1) |