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Table 3 Results of conventional and high-resolution melt PCR analysis for D. immitis DNA in the abdomens and heads of Aedes aegypti collected in south Texas, USA

From: High prevalence of canine heartworm, Dirofilaria immitis, in pet dogs in south Texas, USA, with evidence of Aedes aegypti mosquitoes contributing to transmission

Host

cPCRa

HRM qPCRa

No. of samples tested

Abdomen (%)

Head (%)

No. of samples tested

Head (%)

Cat

22

0 (0)

1 (4.5)

17

1 (5.9)

Dog

92

20 (21.7)

1 (1.1)

83

3 (3.6)

Sheep

3

1 (33.3)

0 (0)

1

0 (0)

Human

57

4 (7.0)

0 (0)

49

0 (0)

Other vertebratesb

10

0 (0)

0 (0)

7

0 (0)

Total

184

25 (13.6)

2 (1.1)

157

4 (2.5)

  1. aNumber of abdomens and heads with bloodmeal testing positive for D. immitis DNA, with the percentage positivity in parentheses
  2. bIncludes chickens, birds, opossums, lizards and pigs