Fig. 4
From: The molecular and functional characterization of ferritins in the hard tick Hyalomma rufipes
a, bSodium dodecyl sulfate–polyacrylamide gel electrophoresis of His-tagged rHrFer1/rHrFer2 purified by nickel NTA affinity chromatography. Protein molecular weight marker (lane M); cell lysate excluding isopropyl β-d-1-thiogalactopyranoside (lane C); fraction washed from Ni–NTA with 20 mM imidazole (lane 1), 50 mM imidazole (lane 2), 100 mM imidazole (lane 3), 200 mM imidazole (lane 4), 250 mM imidazole (lane 5), and 500 mM imidazole (lane 6); Trx tag protein (lane 7)