Skip to main content

Table 1 Primer sequences

From: Comparative analysis of the anticoagulant activities and immunogenicity of HSC70 and HSC70TKD of Haemaphysalis flava

Primer names

Primer sequencesa

HSC70-f-BamH

5′—cgGGATCCGCGAAGGTGCCCGCAATT -3′

HSC70-r-XhoI

5′—ccgCTCGAGATCCACTTCTTCAATTGTGGGCCCACT -3′

TKD-r

5′—AAGGTTCGAGCTGAGCGGCATTCCTC -3′

TKD-f

5′—AATGCCGCTCAGCTCGAACCTTCCGAGCA -3

  1. aThe lowercase letters of primer HSC70-f-BamH and HSC70-r-XhoI are protected bases. The underlined sequences of primer HSC70-f-BamH and HSC70-r-XhoI are cleavage sites of restriction enzymes BamH and XhoI. The letters in primer TKD-r and TKD-f in bold font represent mutation sites