Comparative analysis of the anticoagulant activities and immunogenicity of HSC70 and HSC70TKD of Haemaphysalis flava

Table 2 Determination of serum titer by indirect competitive enzyme-linked immunosorbent assay

Sample		Various dilutions of positive sera								Negative	Blank	Titer
Group	Immunization times	1000	2000	4000	8000	16,000	32,000	64,000	124,000	Negative	Blank	Titer
rHSC70	First	3.826	3.499	3.127	2.126	1.296	0.850	0.576	0.493	0.961	0.146	8000
	Second	3.608	3.472	3.221	2.802	1.932	1.664	1.431	1.375	0.785	0.152	32,000
	Third	3.798	3.723	3.768	3.723	3.492	2.974	2.570	1.946	0.677	0.083	124,000
rHSC70^TKD	First	2.865	1.500	0.807	0.471	0.250	0.174	0.158	0.216	0.271	0.147	4000
	Second	2.993	2.272	2.301	0.894	0.657	0.349	0.228	0.195	0.265	0.133	16,000
	Third	3.244	3.188	3.056	2.205	0.963	0.713	0.656	0.567	0.289	0.131	124,000

rHSC70 Recombinant heat shock cognate 70, rHSC70^TKD Recombinant protein HSC70 (TKD peptide mutation)