Fig. 3

RNA expression profile of cry2 gene, over 24 h (LD12:12), in tissues of Aedes aegypti male mosquitoes. Relative RNA abundance of cry2 in (a) heads,  (b) abdominal tips, and (c) antennae of Ae. aegypti males. Gene expression was determined by quantitative real-time PCR, and graphs were generated from the average of three independent experiments. The y-axis shows the relative RNA abundance, and the x-axis shows the Zeitgeber time (ZT). The gray bar represents the light phase, and the black bar represents the dark phase. Asterisks below the x-axis represent expression differences among high and low values (peaks and troughs), according to Tukey’s a posteriori test. Standard errors are not displayed here for visual ease; instead, they are shown in Additional file 1: Table S1