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Table 1 Nuclear marker profiles of Fasciola flukes used in this study

From: Development of novel DNA marker for species discrimination of Fasciola flukes based on the fatty acid binding protein type I gene

Species

Country

Sample number

FABP type I

(multiplex PCR)

pepck

(multiplex PCR)

pold

(PCR–RFLP)

Fh

Fg

Fh/Fg

Fh

Fg

Fh/Fg

Fh

Fg

Fh/Fg

F. hepatica

Afghanistan [8]

92

92

0

0

85

0

7

92

0

0

Algeria [9]

68

68

0

0

49

1

18

68

0

0

Peru [14] a

114

114

0

0

108

1

5

114

0

0

Spain [11]

196

196

0

0

169

1

26

196

0

0

Subtotal

 

470

470

0

0

411

3

56

470

0

0

F. gigantica

Indonesia [15]

60

0

60

0

0

60

0

0

60

0

Malaysia [20]

36

0

36

0

0

36

0

0

36

0

Nigeria [12]

172

0

172

0

0

172

0

0

157

15

Pakistan [17]

49

0

49

0

0

49

0

0

49

0

Uganda [18]

292

0

292

0

0

292

0

0

292

0

Subtotal

 

609

0

609

0

0

609

0

0

594

15

Hybrid

Japan [16] b

201

0

0

201

0

0

201

0

0

201

Bangladesh c

32

0

0

32

0

0

32

0

0

32

Subtotal

 

233

0

0

233

0

0

233

0

0

233

Total

 

1312

470

609

233

411

612

289

470

594

248

  1. aSeventy-eight Fasciola flukes were used in the previous study. The remaining samples were analyzed in the present study
  2. bThe results of pold were obtained in the present study
  3. cAnalyzed in the present study