Fig. 2

High indole concentrations elicit both depolarization and hyperpolarization currents. a Representative current traces of AaegOR8-Orco activation by 8-s-long stimulations of (R)-1-octen-3-ol alone or increasing indole concentrations (10^–6–10^–2 M). Arrowheads above the traces indicate the onset of the odorant stimulus, black arrowheads represent (R)-1-octen-3-ol and red arrowheads represent indole. b Representative current trace of AaegOR8-Orco activation by 10^–7 M (R)-1-octen-3-ol (black arrowhead) and by 10^–2 M indole (red arrowhead). Statistical significance was determined using the Wilcoxon–Mann–Whitney test (n = 7). c (R)-1-Octen-3-ol was required to elicit significant indole-induced hyperpolarization currents. Nanomolar concentrations of (R)-1-octen-3-ol induced larger indole-induced depolarization currents than indole alone. Representative traces are shown above. Statistical significance was determined using a t-test (n = 7–12)