Fig. 5
Indole reduces anemotactic behavior induced by CO2 and 1-octen-3-ol. a Schematic of the flight tunnel. The ROI was equally divided into three sections represented by differentially shaded volumes (ROI-1, -2, -3). b Kernel density estimations of mosquito coordinates within the first 3 min of odorant-induced flight in ROI-1, -2 and -3. Statistical significance is given in parentheses (P ≤ 0.05). c Bird’s-eye view of the collective flight trajectories elicited by the three odor treatments. Each visible dot represents a trajectory coordinate. Speed levels along the trajectory are color-coded according to the speed index (mm/s). Graded areas below the X-axes represent the three sections of the ROI (1, 2, 3). d Violin plot of mosquito speed in ROI-1, -2 and -3 in response to CO2. e Violin plot of mosquito speed in ROI-1 and -2 as a function of the odor treatments. f Violin plot of upwind velocity in ROI-1 and - as a function of the odor treatments. g Violin plot of tortuosity in ROI-1 and -2 as a function of the odor treatments. In all violin graphs (e–g), the CO2 treatment is colored green, the CO2 + 1-often-3-ol treatment is colored blue and the CO2 + 1-octen-3-ol + indole treatment is colored maroon. CO2 concentration: 600 ppm; 1-octen-3-ol concentration: 168 mg; indole concentration: 14.625 mg. ROI, Region Of Interest. Different lowercase letters above all violin graphs indicate significant difference at P ≤ 0.05). Median values (speed, velocity and tortuosity) are provided below each violin graph