Fig. 4

Detection of HBsAg, HBeAg and HBV DNA copies in the supernatant of HepG2.2.15 cells. HepG2.2.15 cells were incubated with ETV, CsTPs, a mixture of ETV and CsTPs (ETV+CsTP), a mixture of ETV and BSA (ETV+BSA) or a mixture of ETV and CsTPs, respectively pretreated with 0.1, 1 or 10 μg/ml PZQ (ETV+CsTP[PZQ]). In the supernatants of HepG2.2.15 cells, levels of HBsAg (a) and HBeAg (b) were detected by electrochemiluminescence immunoassay, and HBV DNA copies (c) were detected by Q-PCR, at the indicated time points (12, 24 and 48 h). Data were presented as mean values ± SEs. Analysis by one-way ANOVA test indicated significant differences among the different groups at ***P < 0.001 vs the ETV+CsTP group. BeAg, HBV e-antigen; HBsAg, HBV surface antigen; ETV, entecavir; PZQ, praziquantel