Fig. 3

SDS-PAGE analysis of purified recombinant SmM28B and FhM28B. Lanes 1 through 5 show HALO-SmM28B (123 kDa), HALO-SmM28B (E439M) (123 kDa), His-SmM28B (85 kDa), FhM28B (83 kDa), and HALO-FhM28B (E413M) (126 kDa), respectively. Calculated molecular masses of individual constructs are shown in brackets. His-SmM28B was expressed in E. coli, while the remaining constructs were expressed in HEK-293FT cells as fusion proteins with the N-terminal TEV-cleavable His-Strep-HALO tag. For FhM28B, the N-terminal tag was cleaved off by TEV protease. Proteins were separated by reducing SDS-PAGE and stained by Coomassie Brilliant Blue R-250