Fig. 2

Changes in B7-H4 expression on decidual macrophages (dMφ) and the effect of these changes on phagocytic activities during Toxoplasma gondii infection. a B7-H4 expression levels on human CD14⁺ dMφ in uninfected, infected and B7-H4 neutralized infected groups detected by flow cytometry. b B7-H4 expression on mouse F4/80⁺ dMφ detected in uninfected, infected and B7-H4^-/- infected mice by flow cytometry. c Fluorescence microscopy images of the number of phagocytic dMφ in the uninfected, infected and B7-H4 neutralized infected groups. Scale bar: 50 µm. d Flow cytometry results showing the phagocytotic capacity of dMφ in the uninfected, infected and B7-H4 neutralized infected groups. Data are presented as mean ± SD, with at least 6 pregnant mice or human samples in each group assayed individually by the unpaired t-test. Asterisks indicated significant differences between groups at *P < 0.05, **P < 0.01. IgG-FITC, immunoglobulin G-fluorescein isothiocyanate complex