Fig. 4

Reduction of B7-H4 expression on dMφ with T. gondii infection resulted in changes in the expression iNOS and Arg-1. a Levels of Arg-1 produced by dMφ in uninfected, infected and B7-H4^-/- infected mice analyzed by flow cytometry. b Flow cytometry analysis of iNOS produced by dMφ in uninfected, infected, and B7-H4^-/- infected mice. c Western blotting assay of B7-H4, iNOS and Arg-1 protein levels in uninfected, infected and B7-H4 neutralized infected groups of purified human macrophages. Data are presented as the mean ± SD, with at least 6 pregnant mice or human samples in each group assayed individually by the unpaired t-test. Asterisks indicate significant differences between groups at *P < 0.05, **P < 0.01. Arg-1, Arginase-1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; iNOS, inducible nitric oxide synthase