Fig. 5

Reduction of B7-H4 expression on dMφ with T. gondii infection resulted in changes in the expression of TNF-α and IL-10. a-c Expression level of IL-10 and TNF-α in F4/80⁺ dMφ in uninfected, infected and B7-H4^-/- infected mice detected by flow cytometry. d, e Western blotting assay of TNF-α and IL-10 levels in CD14⁺ dMφ of the uninfected, infected and B7-H4 neutralized infected groups. f, g Representative immunofluorescent photographs of B7-H4, TNF-α and IL-10 of CD14⁺ dMφ in the uninfected, infected and B7-H4 neutralized infected groups. Scale bar: 50 µm. Data are presented as the mean ± SD, with at least 6 pregnant mice or human samples in each group assayed individually by the unpaired t-test. Asterisks indicate significant differences between groups at *P < 0.05, **P < 0.01. DAPI, 4′,6-Diamidino-2-phenylindole; IL, interleukin; TNF, tumor necrosis factor