Fig. 7

Adoptive transfer of dMφ from wild-type mice alleviated the adverse pregnancy outcomes and reversed the dysfunction of dMφ caused by T. gondii infection. a-c Pregnancy outcomes: fetuses and placentas in the three groups. d Comparison of placenta, fetal weight and rate of abnormal fetuses in pregnant mice in the three groups. e Proportion of CFSE⁺ macrophages in the placenta of transplanted mice. f Level of B7-H4 on the surface of dMφ in the three groups. g, h Flow cytometry was used to detect the expression of CD86 and CD206 in the three groups. i Detection of the expressions of Arg-1 and iNOS in the three groups by flow cytometry. j, k Detection of the expressions of IL-10 and TNF-α in the three groups by flow cytometry. Group 1, B7-H4^-/- infected mice; group 2, B7-H4^-/- infected mice after adoptive transfer of B7-H4^-/- dMφ; group 3: B7-H4^-/- infected mice after the adoptive transfer of WT dMφ. Data are presented as the mean ± SD, with at least 6 pregnant mice or human samples in each group assayed individually by the unpaired t-test. Asterisks indicate a significant difference between groups at *P < 0.05, **P < 0.01