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Fig. 1 | Parasites & Vectors

Fig. 1

From: Aedes aegypti Malpighian tubules are immunologically activated following systemic Toll activation

Fig. 1

Experimental overview. a The workflow of our experimental setup is illustrated with some mosquito tissues labeled: midgut (mg), fat body (fb), hemocytes (hc) and Malpighian tubules (Mt). Double-stranded RNA (dsRNA) targeting the Cactus gene was injected into the hemocoel and 5 days later RNA sequencing was performed on whole mosquitoes and dissected Malpighian tubules and proteomics was performed on the hemolymph. b The fitness cost of dsCactus treatment was estimated by scoring survival after 5 days. Significantly more individuals from the dsCactus treatment (blue) died compared to the dsLacZ control (gray). Asterisks indicate a Fishers exact test P < 0.0001. Cactus, Negative regulator of the Toll immune pathway in Aedes aegypti; LacZ, Escherichia coli β-galactosidase gene

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