Fig. 2

Comparisons of transcriptional responses of dissected Malpighian tubules and whole mosquitoes following Toll activation. a PCA of RNA-sequencing of whole mosquitoes (squares) and dissected Malpighian tubules (triangles) from both dsLacZ (blue) and dsCactus (black) treatments was visualized for global variation. Numbers indicate biological replicates. b Differentially expressed genes between dsCactus and dsLacZ treatments were compared between whole bodies and Malpighian tubule-specific transcriptomes. c, d Volcano plots were made to visualize the variation in gene expression comparing dsLacZ versus dsCactus in the Malpighian tubules (c) or in whole mosquitoes (d). Red symbols indicate genes that were significantly differentially expressed. DEG, Differentially expressed genes; PC, principal component; PCA, principal component analysis