Fig. 3

Gene set enrichment analysis (GSEA) of Malpighian tubule transcriptional responses to Toll activation. GSEA was performed with six manually curated gene sets which showed significant associations in our Malpighian tubule transcriptomic comparisons. Each panel represents the association of one gene set (CLIPs, SRPNs, AMPs, CTLs, vATPases and PPOs) where the y-axis shows the ES, and the x-axis shows the log₂FC rank of all genes detected in the transcriptome. Black ticks represent where genes in the respective gene list fall on the continuum of ranked genes. ES and the corrected P-value (FDR) for each gene set are shown. ES scores indicate genes enriched in those that are upregulated (positive ES) or downregulated (negative ES) following dsCactus treatment. Full statistical description of all lists can be found in Additional file 5: Table S5. ES, Enrichment score; FDR, false discovery rate