Table 1 Information on simple sequence repeat locui and corresponding primers used in this study
Target SSR locus | Motif | Motif length (bp) | SSR start | SSR end | SSR length (bp) | Motif count | Target length (bp) | Primers |
|---|---|---|---|---|---|---|---|---|
T_01 | GCT | 3 | 76 | 87 | 12 | 4 | 202 | F: CTCGCTGCAAAATCTCCAGC |
R: GAATGGGCTGGCCGTTTAAA | ||||||||
T_02 | CAC | 3 | 86 | 97 | 12 | 4 | 205 | F: CGATGCTGAGGGCGATTTTC |
R: CGACTCCAGACTCTCTACCGA | ||||||||
T_04 | ACT | 3 | 91 | 108 | 18 | 6 | 217 | F: TCCAAAATATTATGCTTTGTCGGCA |
R: GGGCAAACAAGTCCATTTGGT | ||||||||
T_05 | GCT | 3 | 67 | 84 | 18 | 6 | 169 | F: GAGATGATTGCGTCCATGGC |
R: AGAAGCCCATGTCATCGTCG | ||||||||
T_08 | GCAC | 4 | 30 | 41 | 12 | 3 | 131 | F: CGGCTTGGTACAGCCTTCAA |
R: TCGATGCTGCTCTGATTGCT | ||||||||
T_09 | GCAT | 4 | 41 | 52 | 12 | 3 | 138 | F: AGAAATCAGCAGCACCAACA |
R: CGAAAGAGGGGACAGAGCAA | ||||||||
T_11 | CAAC | 4 | 68 | 79 | 12 | 3 | 176 | F: GCCCGAATACCGTCGTTTTG |
R: AGTTGGAGCGGATCACCATG | ||||||||
T_12 | GTGC | 4 | 77 | 96 | 20 | 5 | 148 | F: GCAAACACAAGGCTTCCCAG |
R: GGTCTCTGGTACGTTTGCCA | ||||||||
T_14 | AGA | 3 | 82 | 93 | 12 | 4 | 168 | F: TTGCGCAGACGATAGACAGG |
R: ACGCTGGCTTGTACTCAACA | ||||||||
T_18 | GAT | 3 | 46 | 57 | 12 | 4 | 159 | F: CGACCCGGATCCAAACTACC |
R: GGATCGCCCGTATGATTCCC | ||||||||
T_19 | TGCA | 4 | 78 | 89 | 12 | 3 | 241 | F: TGCCTCGATTTGGTAAGCACT |
R: CTCTGCTTAAGCGACCGTGA | ||||||||
T_21 | GAT | 3 | 55 | 66 | 12 | 4 | 163 | F: GATGCAGGTGACGAGGATGA |
R: GTTTGCTCCCCTCTCCTTCC | ||||||||
T_23 | GGA | 3 | 49 | 60 | 12 | 4 | 171 | F: GCTGGAGAATGCCACTCACA |
R: GCTTCCTCATTCGACTCCGT | ||||||||
T_24 | ACC | 3 | 80 | 91 | 12 | 4 | 171 | F: ACCGTTACAGACAACTGAACCA |
R: AGCTCAACTCGTGAATCCGG | ||||||||
T_27 | CGA | 3 | 54 | 65 | 12 | 4 | 203 | F: GTCTGGGAAGATGGCGTTGA |
R: TCTGTGAGCAAGTGAAGGGC | ||||||||
T_30 | GCA | 3 | 86 | 100 | 15 | 5 | 203 | F: ACCGGCATCAACATCAGTCA |
R: GTAGTCCTCGGGGTGTTGTG | ||||||||
T_31 | CCT | 3 | 83 | 94 | 12 | 4 | 185 | F: CGTCACCAAATTGAACAGCGT |
R: TTCAGCACCATTTTGCAGGC | ||||||||
T_32 | GAG | 3 | 38 | 55 | 18 | 6 | 166 | F: GGAGATGCGATTTCCGGGTT |
R: TCCAACGCAGATGCAACCTA | ||||||||
T_35 | CAG | 3 | 95 | 109 | 15 | 5 | 232 | F: CACAGTTCAGGCTGGCAGTA |
R: GGTCTGAGCGTCCATGTGAT | ||||||||
T_36 | GAG | 3 | 74 | 88 | 15 | 5 | 173 | F: CACCCCTGGAGGTCTGGATA |
R: GGTCGATTTCGCATTCAGCC | ||||||||
T_38 | TTC | 3 | 99 | 113 | 15 | 5 | 232 | F: TTCCCTCCTACAGCGAACCA |
R: GTAGCCTACGACGCCATCTC | ||||||||
T_39 | AGC | 3 | 30 | 41 | 12 | 4 | 157 | F: TTTCGCCTCCTGCACCAG |
R: ACTGGCGAGCATGCAGAG | ||||||||
T_40 | CGA | 3 | 58 | 72 | 15 | 5 | 160 | F: CGCTAAGCCGGTTGTTGC |
R: CGCCAGGTCTCCGTGAAG | ||||||||
T_41 | GCA | 3 | 86 | 103 | 18 | 6 | 211 | F: AAAAGGTGCGTCGTATCCGT |
R: TGCGGCTGACTGTACATCAA | ||||||||
T_42 | TGG | 3 | 79 | 93 | 15 | 5 | 244 | F: CCCAACTGTATCCCCATCGG |
R: AGTAAGCTTCTGCGCCATGA | ||||||||
T_43 | GAG | 3 | 77 | 91 | 15 | 5 | 264 | F: TACTGGTCTCTCGGGCAAGA |
R: GGTCCGTAGTCGTCGTCATC | ||||||||
T_44 | TCC | 3 | 118 | 138 | 21 | 7 | 215 | F: CGCCTCTGGAAGAAGAAGGG |
R: CGTTTGCGCCAGTAACTCTC | ||||||||
T_45 | AGAC | 4 | 54 | 65 | 12 | 3 | 164 | F: CGTAAGCGTTGGAGGAAGGT |
R: CGACCAGAGTTCACCTTCCC | ||||||||
T_48 | TCC | 3 | 102 | 113 | 12 | 4 | 209 | F: TCCACGCCAAGGAGAATGAC |
R: CAGGGGCTTGTAGTGACCG | ||||||||
T_49 | GGA | 3 | 113 | 124 | 12 | 4 | 205 | F: CGTCCGACAGTTGGCAGAAA |
R: CCACACGCGTTTTCACAGTC | ||||||||
T_55 | GAAA | 4 | 61 | 72 | 12 | 3 | 213 | F: TGTGACATAATGGTCCGTGTCA |
R: CCGACCGTTAAGCTGGACAA | ||||||||
T_57 | ACG | 3 | 53 | 70 | 18 | 6 | 209 | F: TGATATGCGAGCAACGGTGT |
R: GGCGAGGAAGTTGACACGAT | ||||||||
T_59 | ACG | 3 | 90 | 101 | 12 | 4 | 209 | F: CGGTCATCTTCCTGCATCGA |
R: TGTGTGATCAGCGACCAGTG | ||||||||
T_60 | CTT | 3 | 75 | 86 | 12 | 4 | 208 | F: GTCCTCAGCACCTGGAACAT |
R: GCCACTCAAGGGTAGGTTGT | ||||||||
T_61 | GAT | 3 | 84 | 95 | 12 | 4 | 233 | F: CGCAAGCCGTTCAGTACAAA |
R: CAGACCCAGACCGCAGAC | ||||||||
T_62 | ATG | 3 | 83 | 97 | 15 | 5 | 173 | F: GGTGAGAGGGCTGAAACACA |
R: CGCTCTACAAGGGTCTCGTC | ||||||||
T_63 | CTC | 3 | 87 | 107 | 21 | 7 | 173 | F: AGCTGTACAATTGCCGTGGA |
R: TCGACTGCCATCCCTCAAAC | ||||||||
T_64 | GCA | 3 | 106 | 117 | 12 | 4 | 167 | F: CAAAGCCCTGTCTCCGGAG |
R: GGGGACTGGAGAGGCAGA | ||||||||
T_65 | CCA | 3 | 77 | 91 | 15 | 5 | 207 | F: CTGCGACAGTTGCCTCCTTA |
R: CGGAAACTCAGTGTGTGGCA |