Fig. 1

a Multiple sequence alignment of DimPgp-3, DimPgp-10 and DimPgp-11 amino acid sequences. The protein regions chosen as the antigenic determinant are highlighted in red. Visualization was prepared using the ExPASy BoxShade (https://embnet.vital-it.ch/software/BOX_form.html). b Two-dimensional visualization of DimPgp-11. The sequence of the transmembrane domains (TMDs), TMD1 (residues 64–354) and TMD2 (residues 726–1012), are framed in red. The nucleotide binding domains (NBDs), NBD1 (residues 389–625) and NBD2 (residues 1045–1218), are framed in blue. Putative N-glycosylation motifs (Asn 100, 134, 138, 478, 663, 706, 1051, 1067, 1161) are highlighted in dark green. The peptide region chosen as the antigenic determinant, the first extracellular loop of the first transmembrane domain, is highlighted in light-green diamonds. Visualization was prepared using the Protter interactive protein visualization tool. DimPgp 3, 10, 11, Dirofilaria immitis P-glycoprotein 3, 10, 11, respectively;