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Fig. 3 | Parasites & Vectors

Fig. 3

From: Genetic polymorphism, constitutive expression and tissue localization of Dirofilaria immitis P-glycoprotein 11: a putative marker of macrocyclic lactone resistance

Fig. 3

 Immunofluorescence assay of DimPgp-11 in Dirofilaria immitis mf by confocal laser microscopy. DimPgp-11 specific signals detected in proximity to the ESP, excretory cell and faintly within the body wall with primary DimPgp-11 antigen affinity antibody and the secondary AlexaFluro488 antibody (green). Counterstaining of Actin with Rhodamine-Phalloidin (red), and cell nuclei with Dapi (blue) distinguished major anatomical features such as the mouth, excretory pore, excretory cell, inner body, anus, and tail in merged immunofluorescence images. a DimPgp-11 localization in Worm 1 and b DimPgp-11 localization in Worm 2. c Non-primary antibody control. The non-primary antibody control was incubated in antibody diluent rather than the primary DimPpg-11 antigen affinity antibody and then incubated with secondary AlexaFluro488 antibody. The absence of immunostaining confirms the specificity of the DimPgp-11 signal observed in Worm 1(a) and 2(b)

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