Table 1 PCR identification of detected cardio-pulmonary parasites

A. abstrusus, T. brevior, A. chabaudi

ITS1-5.8S-ITS2

NC5, NC2 (0.2 µM each)^a

5.0 µl^a/25 µl^a

95 °C^a for 3 min^a; 40 ×^a 94 °C for 30 s, 55 °C for 30 s, 72 °C for 90 s^a; 72 °C^a for 10 min^a

~ 1400 bp

[60]

Capillaria spp.

Cox 1 mtDNA

COIFmod, COIRmod (1.0 µM each)

5.0 µl^a/50 µl

95 °C for 3 min^a; 40 × 94 °C for 30 s, 54 °C for 60 s, 72 °C for 60 s; 72 °C for 10 min

400 bp

[39]

18S rRNA

18S 965, 18S 1573R (1.0 µM each)^a

5.0 µl^a/50 µl

95 °C^a for 3 min^a; 40 ×^a 94 °C for 30 s, 53 °C^a for 30 s^a, 72 °C^a for 60 s^a; 72 °C for 10 min^a

620 bp

[61]