Fig. 1

Histopathological alterations in S. japonicum-infected liver at 0, 4, 7, and 9 weeks. a Schematic diagram of mice infected with S. japonicum cercariae and experiments conducted at different time points after infection. b Anatomy photographs of the liver were taken with a camera. c Representative images of egg granuloma stained by H&E in paraffin-embedded liver tissues (scale bars:100 μm). d The area of egg granuloma in the liver was measured using Image-Pro Plus 6.0. e Representative images of collagen deposition stained with Masson’s trichrome in paraffin-embedded liver tissues (scale bars:100 μm). f The percentage of collagen area was observed by Image-Pro Plus 6.0. Data are presented as mean ± SD (n = 15). ***P < 0.001, compared with the 0 wpi group. ^#P < 0.05, ^###P < 0.001 compared with the 7 wpi group