Fig. 2

The expression of α-SMA, collagen I, and collagen III in primary HSCs of S. japonicum-infected mice at 0, 4, 7, and 9 weeks. a–c Messenger RNA levels of α-SMA, collagen I, and collagen III were determined by RT-qPCR. d The expression levels of α-SMA, collagen I, and collagen III were evaluated by Western blot (e–g), and the densitometric analysis was normalized to the level of endogenous control (β-actin) in primary HSCs. Data are presented as mean ± SD (n = 3). *P < 0.01, ***P < 0.001, compared with the 0 wpi group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 compared with the 7 wpi group