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Fig. 3 | Parasites & Vectors

Fig. 3

From: Effects of different drugs and hormone treatment on Toxoplasma gondii glutathione S-transferase 2

Fig. 3

Progesterone causes abnormal localization of secretory protein transporter VPS10, microneme protein M2AP and MIC2. A Compared with the control group, the localization of Rab5, Rab6 and Rab7 was not affected by P4 treatment, while VPS10 displayed more ring localization (white arrow); target proteins were stained with anti-mouse FLAG antibody; GAP45, anti-rabbit antibody; Hoechst (blue); scale bar, 2 μm; B VPS10 showed two localization forms: a circular trajectories and b short-bar shape. c This localization was independent of whether the parasite was in a divided state or not; d the proportion of ring trajectory localization of VPS10 under P4 was significantly increased; VPS10, anti-mouse FLAG antibody; IMC1, anti-rabbit antibody; D P4 alters the localization of M2AP, which is continuous in the cytoplasm near the outline of the parasite, and MIC2 is more likely to accumulate on the parietal membrane of the parasite, while GRA7 is unaffected; FLAG, anti-mouse antibody, GAP45, anti-rabbit antibody; Hoechst (blue); scale bar, 2 μm

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