Fig. 4

Direct feeding with CAP-containing sugar reduced the laid eggs and inhibited the TOR signaling pathway. A CAP-containing sugar solution feeding significantly reduced the number of eggs laid by female mosquitoes. The red horizontal line represents the median count of laid eggs. B, C, D Comparisons of the hatching, pupation, and emergence rates between the control and CAP groups. E Real-time PCR results showed that feeding adult mosquitoes with CAP-containing sugar solution significantly decreased the mRNA levels of the AsAkt, AsTOR, AsS6K, and AsVg genes compared with those in the control group. F The western blot results showed that the protein levels of p-AKT, p-TOR, p-S6K, and Vg relative to the reference protein β-actin were significantly lower in the CAP group than in the control group. Data analysis was confirmed by t-test. **P < 0.01; ***P < 0.001; ns, no significant difference