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Fig. 1 | Parasites & Vectors

Fig. 1

From: Oral immunization of mice with recombinant Lactobacillus plantarum expressing a Trichinella spiralis galectin induces an immune protection against larval challenge

Fig. 1

The designed immunization scheme and assay protocol. A total of three oral immunizations were given, and five mice of each group were euthanized for assays of intestinal sIgA and cytokines at weeks 0, 2, 4, 6, 7 and 11 after the first immunization. Serum-specific anti-Trichinella spiralis galectin (Tsgal) antibodies (total IgG, IgG1 and IgG2a) were measured by indirect enzyme-linked immunosorbent assay [ELISA] using recombinant Tsgal (rTsgal) at 2 weeks following each immunization and at weeks 1–5, respectively, following challenge. After being challenged with 300 T. spiralis muscle larvae, 10 mice of each group were sacrificed at weeks 7 and 11 after vaccination (i.e. 7 and 35 days post infection [dpi), and adults worms and muscle larvae were recovered to assess the protective efficacy of recombinant NC8-Tsgal/α-lactose against T. spiralis challenge. Histopathological changes of the intestine and muscles from infected mice were examined at 1 and 5 weeks after challenge (i.e. 7 and 35 dpi). Samples were collected at the time-points indicated in the figure. Ig, Immunoglobulin; IL, interleukin; IFN, interferon; sIgA, secretory (mucosal) IgA

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Parasites & Vectors

ISSN: 1756-3305

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