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Fig. 2 | Parasites & Vectors

Fig. 2

From: Oral immunization of mice with recombinant Lactobacillus plantarum expressing a Trichinella spiralis galectin induces an immune protection against larval challenge

Fig. 2

Expression of Tsgal on recombinant NC8-Tsgal bacterium surface by the immunofluorescence test (IFT) and western blotting. a Green fluorescence staining was observed on the surface of Lactobacillus plantarum strain NC8-Tsgal (NC8-Tsgal) by IFT using anti-rTsgal serum. The bacteria recognized by infection serum (serum collected from mice experimentally infected with T. spiralis ML) as a positive control, and normal serum (serum collected from normal mice before immunization) as the negative control. Scale bar: 5 μm. b Western blotting analysis of Tsgal expression. Soluble proteins of normal NC8 (lanes 1, 3, 5) and NC8-Tsgal (lanes 2, 4, 6) were probed by infection serum (lanes 1, 2), anti-rTsgal serum (serum from mice immunized with rTsgal; lanes 3, 4) and normal serum (lanes 5, 6), respectively. The expressed and recognized Tsgal bands have a molecular weight of about 30.4 kDa

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Parasites & Vectors

ISSN: 1756-3305

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