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Fig. 3 | Parasites & Vectors

Fig. 3

From: Oral immunization of mice with recombinant Lactobacillus plantarum expressing a Trichinella spiralis galectin induces an immune protection against larval challenge

Fig. 3

Serum anti-Tsgal IgG measured by ELISA with rTsgal. Anti-Tsgal IgG levels were assayed 2 weeks after the last immunization in sera of mice immunized with NC8-Tsgal. Serum samples diluted at 1:105 and 1:106, respectively, were measured by ELISA with rTsgal. All serum samples were assayed in duplicate. The data are presented as the OD values of anti-Tsgal IgG levels from 10 vaccinated mice. Forty serum samples (1:100 dilutions) from normal mice were measured as negative serum controls. The cut-off value of ELISA was calculated based on the 2.1-fold the mean OD value of the negative control serum from normal mice. Serum OD values that were greater than the cut-off value were regarded as being positive. The cut-off value (0.24) is shown with a dotted line. OD, Optical density; PBS, phosphate-buffered saline

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